WINSTON-SALEM, NC - Top seed Jo-Wilfried Tsonga, former Wimbledon runner-up Tomas Berdych and defending champion John Isner posted second-round wins Tuesday at the $550,000 Winston-Salem Open, a final U.S. Open tune-up.

Tsonga defeated Thomaz Bellucci of Brazil by a 6-3, 7-6 (7-3) margin on the hardcourts at Wake Forest University.

The Frenchman will face Ukrainian qualifier Sergiy Stakhovsky in the third round. Stakhovsky posted a 6-2, 6-3 victory over 15th-seeded Spaniard Pablo Andujar on Tuesday.

The second-seeded Berdych handled Russian Alex Bogomolov Jr. 6-3, 7-5, while Isner, the No. 3 seed, beat Slovakian Martin Klizan 4-6, 6-3, 7-5.

In other action involving top-8 seeds, No. 4 Ukrainian Alexandr Dolgopolov whipped Taiwan's Yen-Hsun Lu 6-1, 6-3, No. 6 Marcel Granollers of Spain handled American Ryan Harrison 6-4, 6-2, No. 7 American Sam Querrey pasted Colombian Santiago Giraldo 6-3, 6-2 in a match that was suspended on Monday night, and Poland's Lukasz Kubot ousted No. 8 Frenchman Julien Benneteau 2-6, 7-5, 6-3. Benneteau was last year's Winston-Salem runner-up to Isner.

Meanwhile, ninth-seeded Spaniard Feliciano Lopez drilled struggling American Donald Young 6-2, 6-3, Belgian Steve Darcis upended 11th-seeded Denis Istomin of Uzbekistan 6-2, 6-2, and 13th-seeded Austrian Jurgen Melzer outlasted American qualifier Michael McClune 6-2, 2-6, 7-6 (12-10). Young is now a dismal 1-18 in his last 19 matches on tour.

Fourteenth-seeded Argentine David Nalbandian handled Dutchman Robin Haase 6-2, 6-4, while 16th-seeded Finnish veteran Jarkko Nieminen erased German qualifier Benjamin Becker 6-3, 6-3 and will meet Berdych in the round of 16.

Latvian qualifier Ernests Gulbis upset 12th-seeded South African Kevin Anderson 6-4, 7-6 (7-2).

The 2012 Winston-Salem champion will collect $73,930.